Two-time Wimbledon men’s champion Andy Murray and defending women’s champion Marketa Vondrousova are both out of the Grand Slam.
Losing in the first round, Vondrousova is the first female holder to fall at the opening hurdle, since Steffi Graf 30 years ago.
The Czech, who became the first unseeded woman to lift the title last year, earned the far less welcome accolade, after a 6-4 6-2 defeat by Spain's Jessica Bouzas Maneiro.
"Yeah, it just didn't go as planned today," said a downcast Vondrousova, adding that, despite her best efforts, the pressure of defending the title had got to her.
"I feel like even if you don't want to think about it, you just think about it. All the time here, I see posters here and everything, my name everywhere.
"Today, I was really nervous since the start and couldn't shake it off. Also, she was playing good.
"I didn't have many chances to come back into the match or she didn't give me many free points. Yeah, credit to her. I'm going to come back stronger, I hope.
"It was an amazing feeling to go back on Centre Court. Now, it's like mixed feelings.
"I love to be here, I would love to stay longer also. Yeah, today was really tough."
Ranked world No. 83, Bouzas Maneiro had won only one tour-level match all year heading into Wimbledon, but secured her first win across all Grand Slams, when she slapped a backhand down the line.
"It is one of the most important moments of my life," said the Spaniard, as she soaked up the cheers. "I'm surprised with myself to be honest."
Meanwhile, Murray, who won his home Grand Slam in 2013 and 2016, didn't even take the court for his first-round match with Tomas Machac, as he chose to pull out of the singles competition.
Murray, 37, will still play doubles alongside brother Jamie in his farewell appearance at the Grand Slam.
The British star underwent surgery on June 22 to remove a spinal cyst that compressed his nerves, and made him lose control and power in his right leg. He decided he was not fit enough for the demands of singles competition.
"I wanted to sleep on it, make sure I was happy with the decision and give myself the chance, when I woke up, to see if it felt much better," he said, a few hours after his team announced the decision.
"I ran around a bit at home this morning when I got up - it wasn't where I wanted it to be, unfortunately.
"It's probably a few days too soon, but I'm proud I worked extremely hard to give myself a chance to play. It's the right decision."
Murray expects to be competitive, when he and his brother take on Australians Rinky Hijikata and John Peers later this week.
"It's not like we're going to roll over and lose the match," he added. "We've got a good chance of winning.
"Me and Jamie play great doubles together, and if both of us are fit and well on the court, we can definitely win the match."
Reuters
