Over 100,000 people have signed a petition calling on British Prime Minister Boris Johnson to stop the killing of New Zealand-born alpaca Geronimo.
Owner Helen Macdonald, a vet nurse who runs an alpaca farm in Gloucestershire, first brought Geronimo to the United Kingdom from New Zealand four years ago.
However, he has since tested positive for bovine tuberculosis twice, and the Department of Food, Environment and Rural Affairs has ordered he be euthanised, the BBC reported.
But Macdonald believes the tests are returning false-positive results and she took the case to London's High Court - although she lost the appeal and a warrant has now been signed for Geronimo to be killed.
She told BBC she won't break the law when officials come to euthanise Geronimo, but she won't "make it easy".
"I'm not going to be helping them kill an animal that does not need to be killed," she said.
"This is an animal injustice and everyone can relate to it and people are coming from all sectors of farming to support us.
"We can make a difference. Geronimo is the poster boy for doing a better job than what we have done in the past."
Macdonald has received an outpouring of support from the British public with over 105,000 people signing a petition calling for the Prime Minister or Environment Secretary George Eustice to overturn the decision.
On Monday, around 30 protesters also marched on Downing Street in a bid to save the animal.
Campaigners carried signs reading "justice for Geronimo" and "no disease, no evidence, no slaughter".
But a spokesperson for Johnson said the order will not be reversed, The Guardian reported.
"We know how distressing losing animals is for anyone. That's why the environment secretary has looked at this extremely carefully, indeed multiple times over a number of years and interrogated all the evidence. The fact remains that Geronimo has sadly tested positive twice, using a highly specific, reliable and validated test."